Developed a variety of two-hybrid system is mostly based system established by fields et al. these new systems are mainly on the reporter gene, "bait" expression vector "prey" expression vector made some improvements. one of the important improvement is the introduction of additional reporter genes his3 gene, such as the widely adopted. transformed

Objective: joint application of recombinant human epidermal growth factor and basic fibroblast growth factor, to explore the impact of diabetic wound healing process. : select 2001-05/2005-03 guangxi medical university, burns and plastic surgery rehabilitation center treated 29 patients with diabetic foot ulcers in patients voluntarily signed infor

Fibroblast growth factor 7 (fgf7) is a secreted by the stromal cells than skin cells as target cells, the heparin binding and cytokines, can be used with the specific receptor is located in the epithelial cell surface binding, then cause a series of intracellularsignal transduction and gene expression play an important role in the process of epithe

The fgf7 main biological function is to promote the proliferation and migration of epithelial cells, inhibition of apoptosis, play an important role in the epithelial tissue injury and repair. common elevated fgf7 transcriptional level, in the healing process of the kidneys, bladder, intestinal mucosa, cornea and skin damage associated with the rel

1984 tanaka et _1 j obtained after the immunization of chicken embryonic spinal cord membrane a monoclonal antibody, using this monoclonal antibody screening spine dry found, which corresponds to the unique antigen expression in motor neurons and backplane cell surface. so named it the sc1 (spinal o. rd) antigen. it first early stage in motor neuro

Epidermal growth factor (epidermal growth factor, egf) is the earliest discovered growth factor plays an important role in regulating cell growth, proliferation and differentiation. in humans, the epidermal growth factor protein of 53 amino acid residues, 6 one thousand three disulfide bonds, quality ear dayton. epidermal growth factor in combinati

Mostly endogenous infection, urinary tract infection, such as urethritis, cystitis,pyelonephritis. e. coli can also cause peritonitis,cholecystitis, appendicitis. infants, the frail elderly, chronic wasting disease, a large area of burn patients, e. coli can invade the bloodstream, causing sepsis. preterm children, especially newborns within 30 day

The yeast two-hybrid system is an important method widely used in group study of protein-protein interactions. its principle is when the target specific binding of protein and the bait protein, the bait protein binding to the promoter of the reporter gene to start the expression of the reporter gene in a yeast cell, if the detected the expression p

Heat-labile enterotoxin (heatstableenterotoxin, st): stable to heat to 100 ° c for 20 minutes still be destroyed, a small molecular weight, the weak immunogenicity. st can activate intestinal epithelial cells guanylate cyclase, increased intracellular cgmp, change the operation of the liquid in the jejunum part of the intestine effusion caused by

Enterotoxin: enterotoxigenic escherichia coli exotoxin released in the process of growth and reproduction, is divided into two kinds of heat-resistant and heat-labile. heat-labile enterotoxin (heatlabileenterotoxin, lt): the heat-labile, 65 ° c for 30 minutes inactivated. of protein, and the large molecular weight and immunogenicity. by a, b subun