Developed a variety of two-hybrid system is mostly based system established by fields et al. these new systems are mainly on the reporter gene, "bait" expression vector "prey" expression vector made some improvements. one of the important improvement is the introduction of additional reporter genes his3 gene, such as the widely adopted. transformed

1984 tanaka et _1 j obtained after the immunization of chicken embryonic spinal cord membrane a monoclonal antibody, using this monoclonal antibody screening spine dry found, which corresponds to the unique antigen expression in motor neurons and backplane cell surface. so named it the sc1 (spinal o. rd) antigen. it first early stage in motor neuro

Cell adhesion molecules are a class of widely distributed on the cell surface or extracellular matrix, mediates cell-cell or cell and matrix q mutual contact and a combination of a class of molecules, play a role by a receptor ligand corresponding forms, resultingcell-cell and cell matrix or cell-matrix adhesion of cells involved in cell signaling

Enterotoxin: enterotoxigenic escherichia coli exotoxin released in the process of growth and reproduction, is divided into two kinds of heat-resistant and heat-labile. heat-labile enterotoxin (heatlabileenterotoxin, lt): the heat-labile, 65 ° c for 30 minutes inactivated. of protein, and the large molecular weight and immunogenicity. by a, b subun

The yeast two-hybrid system is an important method widely used in group study of protein-protein interactions. its principle is when the target specific binding of protein and the bait protein, the bait protein binding to the promoter of the reporter gene to start the expression of the reporter gene in a yeast cell, if the detected the expression p

Fibroblast growth factor 7 (fgf7) is a secreted by the stromal cells than skin cells as target cells, the heparin binding and cytokines, can be used with the specific receptor is located in the epithelial cell surface binding, then cause a series of intracellularsignal transduction and gene expression play an important role in the process of epithe

E. coli the serotyping basis (ie its antigen) of escherichia coli are mainly three antigens: o antigen is the outermost layer of the cell wall lipopolysaccharide specific polysaccharide, composed by repeating polysaccharide units. the antigens stimulate the body to produce igm class antibodies (appeared earlier and disappeared quickly). k antigen,

A certain dose of exogenous bfgf direct role in the organization of the vascular endothelial cells, promote the proliferation, division and growth, the formation of new blood vessels to connect to accelerate the reconstruction of damaged eardrum tissue microcirculation, improve their blood flow and promote thehealing of traumatic tympanic membrane

Epidermal growth factor is the body of an active substance, consisting of 53 amino living cells, by stimulating epidermal growth factor receptor tyrosine phosphorylation, the skin surface cells to repair hyperplasia, is said to injuries,the loss of the epidermis has an excellent efficacy. its greatest feature is able to promote cell proliferation a

The fgf7 main biological function is to promote the proliferation and migration of epithelial cells, inhibition of apoptosis, play an important role in the epithelial tissue injury and repair. common elevated fgf7 transcriptional level, in the healing process of the kidneys, bladder, intestinal mucosa, cornea and skin damage associated with the rel